Path of Science: International Electronic Scientific Journal

Comparative larvicidal efficacy of chloroform-extracted metabolites of Emericella nidulans with those of conventional chemical larvicides, as well as gas chromatography-mass spectrometry (GC-MS) characterisation of bioactive compounds of E. nidulans, is presented in this work. The chloroform extract showed higher larvicidal activity than the diethyl ether and ethyl acetate extracts, with a 72-hour LC₅₀ of 312 µg/ml, comparable to temephos (LC₅₀ = 287 µg/ml). GC-MS was used to identify 14 secondary metabolites, including phenols, esters, hydrocarbons, and fatty acid derivatives, known for their insecticidal properties. The integrated discussion provides a link among the results obtained using morphological, molecular, and biochemical techniques, confirming E. nidulans as an eco-friendly alternative to chemical larvicides. These results highlight the potential utility of fungal metabolites as sustainable tools in integrated mosquito vector management programmes, especially in malaria-endemic areas where the use of conventional insecticides is hampered by increasing resistance. 

Emericella nidulans; larvicidal activity; Anopheles mosquito; GC–MS analysis; biocontrol; entomopathogenic fungi; integrated vector man-agement

1. Scholte, E., Knols, B. G. J., Samson, R. A., & Takken, W. (2004). Entomopathogenic fungi for mosquito control: A review. Journal of Insect Science, 4(19), 1–24. doi: 10.1673/031.004.1901

2. Benelli, G. (2015). Research in mosquito control: current challenges for a brighter future. Parasitology Research, 114(8), 2801–2805. doi: 10.1007/s00436-015-4586-9

3. Delazar, A., Naseri, M., Nahar, L., Moghadam, S. B., Esnaashari, S., Nazemiyeh, H., & Sarker, S. D. (2007). GC-MS analysis and antioxidant activities of essential oils of two cultivated Artemisia species. Chemistry of Natural Compounds, 43(1), 112–114. doi: 10.1007/s10600-007-0047-8

4. Perumal, V., Kannan, S., Pittarate, S., & Krutmuang, P. (2024). A review of entomopathogenic fungi as a potential tool for mosquito vector control: A cost‐effective and environmentally friendly approach. Entomological Research, 54(3). doi: 10.1111/1748-5967.12717

5. Kweka, E. J., Zhou, G., Munga, S., Lee, M., Atieli, H. E., Nyindo, M., Githeko, A. K., & Yan, G. (2012). Anopheline Larval Habitats: Seasonality and species distribution: A prerequisite for effective targeted larval habitats control programmes. PLoS ONE, 7(12), e52084. doi: 10.1371/journal.pone.0052084

6. Naqqash, M. N., Gökçe, A., Bakhsh, A., & Salim, M. (2016). Insecticide resistance and its molecular basis in urban insect pests. Parasitology Research, 115(4), 1363–1373. doi: 10.1007/s00436-015-4898-9

7. Caraballo, H., King, K., Akhtar, S., & Bentley, S. (2014). Emergency Department Management of Mosquito-Borne Illness: Malaria, Dengue, and West Nile Virus. Emergency Medicine Practice, 16(5), 1–23.

8. Ukpai, O., & Ajoku, E. (2006). The prevalence of malaria in the Okigwe and Owerri areas of Imo State. Nigerian Journal of Parasitology, 22(1). doi: 10.4314/njpar.v22i1.37757

9. World Health Organisation (2005). Guidelines for laboratory and field testing of mosquito larvicides. Retrieved from https://iris.who.int/bitstreams/1cab30dc-a9bd-4df3-9cce-281fd35e316d/download

10. N'Guessan, R., Corbel, V., Akogbéto, M., & Rowland, M. (2007). Reduced efficacy of insecticide-treated nets and indoor residual spraying for malaria control in a pyrethroid resistance area, Benin. Emerging Infectious Diseases, 13(2), 199–206. doi: 10.3201/eid1302.060631

11. Oyewole, I.O., Ogunnowo, A. A., Ibidapo, C. A., Okoh, H. I., Awolola, T. S., & Adedayo, M. A. (2011). Epidemiology of Malaria and the Burden of Insecticide Resistance in Nigeria. Journal of Public Health and Epidemiology, 3(1), pp. 6-12.

12. Goettel, M. S., Koike, M., Kim, J. J., Aiuchi, D., Shinya, R., & Brodeur, J. (2008). Potential of Lecanicillium spp. for management of insects, nematodes and plant diseases. Journal of Invertebrate Pathology, 98(3), 256–261. doi: 10.1016/j.jip.2008.01.009

13. Seye, F., Ndiaye, M., Faye, O., & Afoutou, J. M. (2012). Evaluation of Entomopathogenic Fungus Metarhizium anisopliae Formulated with Suneem (Neem Oil) against Anopheles gambiae s.l. and Culex quinquefasciatus Adults. Malaria Chemotherapy Control and Elimination, 1, 1–6. doi: 10.4303/mcce/235494

14. Lacey, L. A. (2007). Bacillus thuringiensis serovariety israelensis and Bacillus sphaericus for mosquito control. Journal of the American Mosquito Control Association 23(2), 133-163.

15. Jaber, S., Mercier, A., Knio, K., Brun, S., & Kambris, Z. (2016). Isolation of fungi from dead arthropods and identification of a new mosquito natural pathogen. Parasites & Vectors, 9(1), 491. doi: 10.1186/s13071-016-1763-3

16. Quesada-Moraga, E., Ruiz-García, A., & Santiago-Álvarez, C. (2006). Laboratory Evaluation of Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae Against Puparia and Adults of Ceratitis capitata (Diptera: Tephritidae). Journal of Economic Entomology, 99(6), 1955–1966. doi: 10.1093/jee/99.6.1955

17. Amer, A., & Mehlhorn, H. (2006). Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitology Research, 99(4), 466–472. doi: 10.1007/s00436-006-0182-3

18. Kabir, S., Lawal, N., Ganiyu, A. I., & Suleiman, I. (2024). Larvicidal Effect of Spores and Metabolites Extracts of Aspergillus Fumigatus against Culex Mosquito Larvae. UMYU Journal of Microbiology Research (UJMR), 9(3), 550–559. doi: 10.47430/ujmr.2493.060

19. Matasyoh, J. C., Dittrich, B., Schueffler, A., & Laatsch, H. (2010). Larvicidal activity of metabolites from the endophytic Podospora sp. against the malaria vector Anopheles gambiae. Parasitology Research, 108(3), 561–566. doi: 10.1007/s00436-010-2098-1

20. Benserradj, O., & Mihoubi, I. (2014). Larvicidal activity of entomopathogenic fungi Metarhizium anisopliae against mosquito larvae in Algeria. International Journal of Current Microbiology and Applied Sciences, 3(1), 54-62.

21. Huang, Y., Higgs, S., & Vanlandingham, D. (2017). Biological control strategies for mosquito vectors of arboviruses. Insects, 8(1), 21. doi: 10.3390/insects8010021

22. Daniel, J., Silva, A., Nakagawa, D., De Medeiros, L., Carvalho, M., Tavares, L., Abreu, L., & Rodrigues-Filho, E. (2016). Larvicidal Activity of Beauveria bassiana Extracts against Aedes aegypti and Identification of Beauvericins. Journal of the Brazilian Chemical Society. doi: 10.21577/0103-5053.20160253

23. Garrido-Jurado, I., Alkhaibari, A., Williams, S. R., Oatley-Radcliffe, D. L., Quesada-Moraga, E., & Butt, T. M. (2015). Toxicity testing of Metarhizium conidia and toxins against aquatic invertebrates. Journal of Pest Science, 89(2), 557–564. doi: 10.1007/s10340-015-0700-0

24. Bhan, S., Mohan, L., & Srivastava, C. N. (2013). Larvicidal Toxicity of Temephos and Entomopathogenic Fungus, Aspergillus Flavus and Their Synergistic Activity Against the Malaria Vector, Anopheles Stephensi. Journal of Entomology and Zoology Studies, 1(6), 55-60.

25. Hamad, B. S., Khudair, M. W., Kathiar, S. A., & Abdullatif, A. M. (2015). Efficacy of some local isolates of Beauveria bassiana(Bals.) and Metarhizium anisopliae (Met.) in control of mosquito larvae of Culex quinquefasciatus (Say). Baghdad Science Journal, 12(4), 677–683. doi: 10.21123/bsj.2015.12.4.677-683