Bio-Scouring of Cotton using Protease and Pectinase from Bacillus subtilis Isolated from Market Waste

Chidiebere Anab-Atulomah, Ejikeme Nwachukwu

Abstract

The bio-scouring of cotton using protease and pectinase produced from Bacillus subtilis was investigated. Protease and pectinase were produced from Bacillus subtilis in a liquid medium using the submerged fermentation technique. Both enzymes were purified, and their scouring potential was tested on raw cotton fabrics. Pectinase was more effective than protease under optimised conditions. The optimum scouring temperature for both enzymes was between 40 °C and 50 °C, with pectinase bio-scoured fabric showing 15.5% weight loss while protease bio-scoured fabric had 14.3% weight loss. The optimum pH for pectinase scouring was pH 9 with 14.8% weight loss in the fabric, while the optimum pH for protease scoured fabric was pH 7 with 12.3% weight loss in fabric. After 120 minutes of bio-scouring, maximum weight loss was recorded for both pectinase and protease treated fabrics. The application of protease and pectinase for cotton fabric scouring revealed that they could be used as bio-scouring agents to treat textile materials.


10.22178/pos.72-3


Keywords


Bio-scbio-scouring; cotton; Bacillus subtilis; protease; pectinase

Full Text:

PDF


References


Dalvi, P., Anthappan, P., Darade, N., Kanoongo, N., & Adivarekar, R. (2007). Amylase and pectinase from single source for simultaneous desizing and scouring. Indian Journal of Fibre and Textile Research, 32, 459-465.

Badhe, P., Joshi, M., & Adivarekar, R. (2016). Optimised production of extracellular proteases by Bacillus subtilis from degraded abattoir waste. Journal of Bioscience and Biotechnology, 5(1), 29–36.

Badhe, P., Damale, M., & Adivarekar, R. (2017). Bioscouring of wool using protease from bacillus subtilis isolated from abattoir waste. Journal of Microbiology, Biotechnology and Food Sciences, 6(4), 1012–1018. doi: 10.15414/jmbfs.2017.6.4.1012-1018

Bandow, J. E., Brötz, H., & Hecker, M. (2002). Bacillus subtilis Tolerance of Moderate Concentrations of Rifampin Involves the σB-Dependent General and Multiple Stress Response. Journal of Bacteriology, 184(2), 459–467. doi: 10.1128/jb.184.2.459-467.2002

Boylston, E. K., & Usda, J. J. H. (1995). The Primary Wall of Cotton Fibers. Textile Research Journal, 65(7), 429–431. doi: 10.1177/004051759506500711

Deng, A., Wu, J., Zhang, Y., Zhang, G., & Wen, T. (2010). Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001. Bioresource Technology, 101(18), 7100–7106. doi: 10.1016/j.biortech.2010.03.130

Kobayashi, T., Koike, K., Yoshimatsu, T., Higaki, N., Suzumatsu, A., Ozawa, T., … Ito, S. (1999). Purification and Properties of a Low-molecular-weight, High-alkaline Pectate Lyase from an Alkaliphilic Strain ofBacillus. Bioscience, Biotechnology, and Biochemistry, 63(1), 65–72. doi: 10.1271/bbb.63.65

Machado, C. M. M., Oishi, B. O., Pandey, A., & Soccol, C. R. (2004). Kinetics of Gibberella fujikuroi Growth and Gibberellic Acid Production by Solid-State Fermentation in a Packed-Bed Column Bioreactor. Biotechnology Progress, 20(5), 1449–1453. doi: 10.1021/bp049819x

Teli, M., & Adere, T. (2016). Process optimisation for bioscouring of 100% cotton textiles using Box-Behnken design. Advances in Applied Science Research, 7(4), 209– 221.

Mojsov, K. (2012). Microbial Alpha-Amylases and their Industrial Applications: A Review. International Journal of Management, IT and Engineering, 2(10), 583–609.

Mala, B. R., Aparna, M. T., Mohini, S. G., Vasanti, V. D. (1998). Molecular and Biotechnological Aspects of Microbial Proteases. Microbiology and Molecular Biology Review, 62(3), 597–635.

Kavuthodi, B., Thomas, S., & Sebastian, D. (2015). Co-production of Pectinase and Biosurfactant by the Newly Isolated Strain Bacillus subtilis BKDS1. British Microbiology Research Journal, 10(2), 1–12. doi: 10.9734/bmrj/2015/19627

Muthulakshmi, Ch., Gomathi, D., Kumar, D. G., Ravikumar, G., Kalaiselvi, M., & Uma, Ch. (2011). Production, purification and characterisation of protease by Aspergillus flavus under solid state fermentation. Jordan Journal of Biological Sciences, 4(3), 137–148.

Waddell, R. (2002). Bioscouring of cotton: commercial applications of alkaline stable pectinase. AATCC Review, 2, 28–30.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2021 Chidiebere Anab-Atulomah, Ejikeme Nwachukwu

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.