Diagnostic Analysis of Student Misconceptions on Viral and Bacterial Concepts in Introductory Biology Courses

Chinaza Gloria Diala, Sophia Chidera Anaba, Georgina Mary Adu, Conlethann Chiemerie Ohaekwe, Opeyemi Alice Ajayi, Tracy Obofoni Obakhena

Abstract

The fundamental aspect of biology education is not only understanding the difference between viruses and bacteria, but also recognising that this knowledge is essential for everyone worldwide, as public health requires a clear distinction between the two. Even though teachers cover these topics in secondary school, students still confuse the two microorganisms, even at the university level. This study investigates how first-year students in biological sciences understand viruses and bacteria, particularly their structural characteristics, reproduction, treatment options, and roles.

Researchers surveyed 160 first-year biology and microbiology students across three universities in Imo State using a mixed-methods approach. The survey included multiple-choice questions, open-ended responses, and short case scenarios designed to probe both factual knowledge and deeper conceptual understanding. We also conducted follow-up interviews with 20 students to clarify some of their written responses and explore their reasoning in more depth.

Our findings indicate that the majority of students correctly identified viruses as non-living and bacteria as living; however, a high number still believed that antibiotics can treat viral infections. Also, many students felt that viruses are smaller versions of bacteria or that both reproduce in the same way. There were misunderstandings among students regarding the role of bacteria. Many students classified bacteria as pathogenic organisms that cause disease, forgetting that they play beneficial roles in ecosystems, human health, and the microbiome.

Early gaps, the ongoing reduction in science teaching in primary and secondary schools, and the spread of misleading information through the media and in conversation are significant causes of the misconceptions observed among students. This study emphasised the need to implement an effective teaching method for introductory biology courses at all levels, from primary to university, particularly those focusing on theoretical understanding and addressing students' initial beliefs. To help students build a solid foundation and knowledge in advanced biology, these misunderstandings need to be addressed, as they will support decision-making in public health.




Keywords


Viruses; Bacteria; Misconceptions; Biology Education; Public Health; Student Understanding; Science Curriculum

Full Text:

PDF


References


1. Barro, P. (2020). 'Deeply worrying': 92% of Australians don't know the difference between viral and bacterial infections. The Conversation Media Group Ltd. doi: 10.64628/aa.wsyq5ngyj

2. Pickett, S. B., Nielson, C., Marshall, H., Tanner, K. D., & Coley, J. D. (2022). Effects of Reading Interventions on Student Understanding of and Misconceptions about Antibiotic Resistance. Journal of Microbiology and Biology Education, 23(1). doi: 10.1128/jmbe.00220-21

3. Simard, C. (2021). Microorganism education: misconceptions and obstacles. Journal of Biological Education, 57(2), 308–316. doi: 10.1080/00219266.2021.1909636

4. Lane, A. R., Momsen, J. L., & Wiltbank-Chau, L. B. (2025). Positive microbiology: addressing students' knowledge gaps regarding the benefits of microorganisms. Journal of Microbiology and Biology Education. doi: 10.1128/jmbe.00045-25

5. Bracko, M., & Simon, U. K. (2022). Virus-related Knowledge in Covid-19 Times - Results from two Cross-sectional Studies in Austria and Implications for Schools. International Journal of Biological Sciences, 18(4), 1627–1650. doi: 10.7150/ijbs.69224

6. Berg, G., Rybakova, D., Fischer, D., Cernava, T., Vergès, M. C., Charles, T., Chen, X., Cocolin, L., Eversole, K., Corral, G. H., Kazou, M., Kinkel, L., Lange, L., Lima, N., Loy, A., Macklin, J. A., Maguin, E., Mauchline, T., McClure, R., Mitter, B., Ryan, M., Sarand, I., Smidt, H., Schelkle, B., & Schloter, M. (2020). Microbiome definition revisited: old concepts and new challenges. Microbiome, 8(1). doi: 10.1186/s40168-020-00875-0

7. McCullough, A. R., Parekh, S., Rathbone, J., Del Mar, C. B., & Hoffmann, T. C. (2015). A systematic review of the public's knowledge and beliefs about antibiotic resistance. Journal of Antimicrobial Chemotherapy, 71(1), 27–33. doi: 10.1093/jac/dkv310

8. Vallin, M., Polyzoi, M., Marrone, G., Rosales-Klintz, S., Wisell, K. T., & Lundborg, C. S. (2016). Knowledge and Attitudes towards Antibiotic Use and Resistance - A Latent Class Analysis of a Swedish Population-Based Sample. PLoS ONE, 11(4), e0152160. doi: 10.1371/journal.pone.0152160

9. Whitaker-Dowling, P., & Youngner, J. S. (1999). Virus-Host Cell Interactions. In Elsevier eBooks (pp. 1957–1961). doi: 10.1006/rwvi.1999.0343

10. Norval, M. (2012). Virus–cell interactions. In Elsevier eBooks (pp. 82–96). doi: 10.1016/b978-0-7020-4089-4.00022-6

11. Zhu, T., Niu, G., Zhang, Y., Chen, M., Li, C., Hao, L., & Zhang, Z. (2023). Host-mediated RNA editing in viruses. Biology Direct, 18(1). doi: 10.1186/s13062-023-00366-w

12. Diala, C. G., Edobor, O., Edim, P. D., Oraekwe, C. N., Okenwa, C. R., Onyema, A. M., Hammed, S. U., & Onyeaghala, C. A. (2025). Antimicrobial Resistance (AMR) in Low-Resource Settings: A molecular and public health investigation into the role of environmental reservoirs and Over-The-Counter antibiotic use in South-South Nigeria. Path of Science, 11(8), 5008. doi: 10.22178/pos.121-15

13. Coley, J. D., & Tanner, K. (2015). Relations between Intuitive Biological Thinking and Biological Misconceptions in Biology Majors and Nonmajors. CBE – Life Sciences Education, 14(1), 8. doi: 10.1187/cbe.14-06-0094

14. Leonard, M. J., Kalinowski, S. T., & Andrews, T. C. (2014). Misconceptions yesterday, today, and tomorrow. CBE – Life Sciences Education, 13(2), 179–186. doi: 10.1187/cbe.13-12-0244

15. Sánchez‐Angulo, M. (2025). Positive microbiology in the movies. Microbial Biotechnology, 18(9). doi: 10.1111/1751-7915.70085

16. Briggs, A. G., Hughes, L. E., Brennan, R. E., Buchner, J., Horak, R. E. A., Amburn, D. S. K., McDonald, A. H., Primm, T. P., Smith, A. C., Stevens, A. M., Yung, S. B., & Paustian, T. D. (2017). Concept Inventory Development Reveals Common Student Misconceptions about Microbiology. Journal of Microbiology and Biology Education, 18(3). doi: 10.1128/jmbe.v18i3.1319

17. Kaba, H. E. J., Misailovski, M., Brähler, J., Garcia, J. a. B., Artelt, T., Raupach, T., & Scheithauer, S. (2024). Innovative teaching in infection prevention and control and infectious diseases education: testing and investigation of student perceptions. Infection. doi: 10.1007/s15010-024-02332-8

18. Jawad, A. K., & Okab, A. A. (2024). Teachers' Awareness About Control of Communicable Diseases at Primary Schools in Al-Swaira City. Thi-Qar Medical Journal, 25(1).

19. Hartelt, T., Martens, H., & Minkley, N. (2022). Teachers' ability to diagnose and deal with alternative student conceptions of evolution. Science Education, 106(3), 706–738. doi: 10.1002/sce.21705

20. Ogundare, A., Bello, G., Adeoye, G. A., & Sulaiman, M. M. (2024). Remediating students' misconceptions in biology: A review. Custech International Journal of Education, 1(2), 89–102.

21. Saputri, D. A. F., & Widyaningrum, T. (2016). Misconceptions Analysis on The Virus Chapter in Biology Textbooks for High School Students Grade X. International Journal of Active Learning. 1(1), 30-37

22. Novitasari, C., Ramli, M., & Karyanto, P. (2019). Content analysis of misconceptions on bacteria in the biology textbook of high school. Journal of Physics Conference Series, 1157, 022076. doi: 10.1088/1742-6596/1157/2/022076

23. McGenity, T. J., Gessesse, A., Hallsworth, J. E., Cela, E. G., Verheecke‐Vaessen, C., Wang, F., Chavarría, M., Haggblom, M. M., Molin, S., Danchin, A., Smid, E. J., Lood, C., Cockell, C. S., Whitby, C., Liu, S., Keller, N. P., Stein, L. Y., Bordenstein, S. R., Lal, R., Keller, N. P., Stein, L. Y., Bordenstein, S. R., Lal, R., Nunes, O. C., Gram, L., Singh, B. K., Webster, N. S., Morris, C., Sivinski, S., Bindschedler, S., Junier, P., Antunes, A., Baxter, B. K., Scavone, P., & Timmis, K. (2020). Visualising the invisible: class excursions to ignite children's enthusiasm for microbes. Microbial Biotechnology, 13(4), 844–887. doi: 10.1111/1751-7915.13576

24. Ningtyas, L. E., & Asri, M. T. (2021). Profile of 10th Grade High school students' Misconceptions on the virus topic based on the Three-Tier Multiple Choice Diagnostic Test. Berkala Ilmiah Pendidikan Biologi (BioEdu), 10(3), 674–682. doi: 10.26740/bioedu.v10n3.p674-682

25. Oli, M. W. (2023). On the Path to "Positive Microbiology." Journal of Microbiology and Biology Education, 24(2). doi: 10.1128/jmbe.00066-23

26. Rivard, R. S., Winters, M., Lettini, S., & McEliece, M. K. (2024). A customisable series of microbiology lab activities exploring a foodborne outbreak to enhance student recruitment to a biology program. Journal of Microbiology and Biology Education, 25(1). doi: 10.1128/jmbe.00086-23


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Copyright (c) 2025 Chinaza Gloria Diala, Sophia Chidera Anaba, Georgina Mary Adu, Conlethann Chiemerie Ohaekwe, Opeyemi Alice Ajayi, Tracy Obofoni Obakhena

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.