Cycloeucalenone Isolation and Characterisation from Musa Acuminata Colla

Linus Usman Akacha, Kevin Shadrach Ndyakalah

Abstract

The isolation and characterisation of bioactive compound(s) from Musa acuminata colla (banana blossom) as a result, the research aimed to isolate secondary metabolites. Sample Extraction was carried out using the maceration method to obtain ethyl acetate extracts. The Ethyl acetate extract of the Banana blossom flower and bract was used for isolation. Compounds isolation using Column Chromatography, the 260 fractions from the column are subjected to TLC testing to see the separation of the component combination by applying a thin stationary phase supported by an inert backing that yields 30 fractions. The purpose of TLC is to obtain well-defined, well-separated spots. Each component has a retention factor (RF), which combines fractions with the same RF value. The four fractions obtained from TLC activities were analysed using NMR spectroscopy (FT-IR, 1D NMR, 2D NMR and Mass spec), which resulted in the isolation of triterpenes compounds named Cycloeucalenone.


10.22178/pos.113-27


Keywords


Musa acuminata Colla; 1D NMR; 2D NMR; mass spectrometry; FT-IR

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References


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Copyright (c) 2025 Linus Usman Akacha, Kevin Shadrach Ndyakalah

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