Path of Science: International Electronic Scientific Journal

Emerging antibacterial resistance is a great challenge to modern medicine and is an increasing problem in different regions, limiting therapeutic options. Therefore, this study aimed to use the Citrus aurantium extract and assess its effect on bacterial agents. The antibacterial potentials of orange (Citrus aurantium) peel were evaluated on Gram-positive and Gram-negative bacteria. Three solvents, Ethanol, Methanol and Hot water, were used for extraction. The resultant extracts were used on three Gram-positive and five Gram-negative bacteria isolated from FUTA Botanical Garden. These phytochemicals include alkaloids, glycosides, phenols, tannins, saponins, terpenoids and steroids. The agar well diffusion method assessed the antibacterial activity, reflected by the diameter of growth inhibition zones. Results showed that among the solvents used, the extracts exhibited better performance in the order of Ethanol > Methanol > Hot water, which reflects the suitability of solvents for Citrus aurantium peel extraction. It was found that Gram-positive bacteria were more sensitive to the extracts at 200 mg/ml. Among them, Staphylococcus aureus showed the highest sensitivity against extracts from (15.00±1.00 mm to 21.00±1.00 mm). Among the Gram-negative bacteria, Klebsiella spp was susceptible to the 200 mg/ml extracts ranging from (13.00±1.00 mm to 15.00±1.00 mm). This may be attributed to a high concentration of flavonoids and total phenolic content in the ethanolic and methanolic extracts of Citrus aurantium peel.

Antibacterial; Resistance; Gram-positive; Gram-negative; Ethanol; Methanol; Hot water; Sensitivity; Citrus aurantium; Phytochemicals

1. Doughari, J. H., El-Mahmood, A. M., & Tyoyina, I. (2011). Antimicrobial Activity of Leaf Extracts of Senns obtusifolia (L). African Journal of Pharmacy and Pharmacology, 2(1), 7-13

2. Onyeagba, R. A., Ugbogu, O. C., Okeke, C. U., & Iroakasi, .O. (2004). Studies on the antimicrobial effects of garlic (Allium sativum Linn), ginger (Zingiber officinale Roscoe) and lime (Citrus aurantifolia Linn). African Journal of Biotechnology, 3(10), 552–554. doi: 10.5897/ajb2004.000-2108

3. Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. N. d.: Spectrum Books.

4. Kimbi, H. K., & Fagbenro-Beyioku, A. F. (1996). Efficacy of Cymbopogon giganteus and Enantia chrantha against chloroquine resistant Plasmodium yoelii nigeriensis. East African medical journal, 73(10), 636–637.

5. Hammer, K. A., Carson, C. F., & Riley, T. V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6), 985–990. doi: 10.1046/j.1365-2672.1999.00780.x

6. Ejaz, S., Ejaz, A., Matsuda, K., & Lim, C. W. (2005). Limonoids as cancer chemopreventive agents. Journal of the Science of Food and Agriculture, 86(3), 339–345. doi: 10.1002/jsfa.2396

7. Karabıyıklı, Ş., Değirmenci, H., & Karapınar, M. (2014). Inhibitory effect of sour orange (Citrus aurantium) juice on Salmonella Typhimurium and Listeria monocytogenes. LWT - Food Science and Technology, 55(2), 421–425. doi: 10.1016/j.lwt.2013.10.037

8. Swingle, W. T. (1943). The botany of citrus and its wild relatives of the Orange Subfamily (family Rutaceae, Subfamily Aurantioideae). N. d.: University of California Press.

9. Akçalıoğlu, O., Ağçam, E., Polat, S., Uçan, F., Aslan, S., & Akyıldız, A. (2014). Turunç ekşisi üzerine bir araştırma. Geleneksel Gıdalar Sempozyumu, 17(19), 814–818.

10. Karoui, I. J., Wannes, W. A., & Marzouk, B. (2010). Refined corn oil aromatisation by Citrus aurantium peel essential oil. Industrial Crops and Products, 32(3), 202–207. doi: 10.1016/j.indcrop.2010.04.020

11. Pietta, P.-G. (2000). Flavonoids as Antioxidants. Journal of Natural Products, 63(7), 1035–1042. doi: 10.1021/np9904509

12. Stohs, S. J., Preuss, H. G., Keith, S. C., Keith, P. L., Miller, H., & Kaats, G. R. (2011). Effects of p-Synephrine alone and in Combination with Selected Bioflavonoids on Resting Metabolism, Blood Pressure, Heart Rate and Self-Reported Mood Changes. International Journal of Medical Sciences, 8(4), 295–301. doi: 10.7150/ijms.8.295

13. Fukumoto, L. R., & Mazza, G. (2000). Assessing Antioxidant and Prooxidant Activities of Phenolic Compounds. Journal of Agricultural and Food Chemistry, 48(8), 3597–3604. doi: 10.1021/jf000220w

14. Okwu, D. E. (2005). Phytochemicals, Vitamins and Mineral contents of two Nigerian Medicinal Plants. International Journal of Molecular Advanced Science, 1(4), 375–381.

15. Perry, G., Raina, A. K., Nunomura, A., Wataya, T., Sayre, L. M., & Smith, M. A. (2000). How important is oxidative damage? Lessons from Alzheimer's disease. Free Radical Biology and Medicine, 28(5), 831–834. doi: 10.1016/s0891-5849(00)00158-1

16. Cook, N. (1996). Flavonoids?Chemistry, metabolism, cardioprotective effects, and dietary sources. Journal of the European Ceramic Society, 66–76. doi: 10.1016/s0955-2863(95)00168-9

17. Benavente-García, O., & Castillo, J. (2008). Update on Uses and Properties of Citrus Flavonoids: New Findings in Anticancer, Cardiovascular, and Anti-inflammatory Activity. Journal of Agricultural and Food Chemistry, 56(15), 6185–6205. doi: 10.1021/jf8006568

18. Dey, R. A., Underwood, A. L. (1986). Quantitative Analysis. N. d.: Prentice Hall Publication.

19. Adzitey, F. (2015). Antibiotic Classes and Antibiotic Susceptibility of Bacterial Isolates from Selected Poultry; A Mini Review. World s Veterinary Journal, 6(1), 36. doi: 10.5455/wvj.20150853

20. Aminov, R. I. (2010). A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future. Frontiers in Microbiology, 1. doi: 10.3389/fmicb.2010.00134

21. Acar, J. (1997). Broad- and narrow-spectrum antibiotics: an unhelpful categorisation. Clinical Microbiology and Infection, 3(4), 395–396. doi: 10.1111/j.1469-0691.1997.tb00274.x

22. Woodford, N., & Livermore, D. M. (2009). Infections caused by Gram-positive bacteria: a review of the global challenge. Journal of Infection, 59, S4–S16. doi: 10.1016/s0163-4453(09)60003-7

23. Cornaglia, G. (2009). Fighting infections due to multidrug-resistant Gram-positive pathogens. Clinical Microbiology and Infection, 15(3), 209–211. doi: 10.1111/j.1469-0691.2009.02737.x

24. Biemer J. J. (1973). Antimicrobial susceptibility testing by the Kirby-Bauer disc diffusion method. Annals of clinical laboratory science, 3(2), 135–140.

25. Cowan, M. M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12(4), 564–582. doi: 10.1128/cmr.12.4.564

26. Siddique, S., Shafique, M., Parveen, Z., Khan, S. J., & Khanum, R. (2011). Volatile components, antioxidant and antimicrobial activity of Citrus aurantium (bitter orange) peel oil. Pharmacologyonline, 2, 499–507.

27. Tumane, P. M., Meshram, V., & Wasnik, D. D. (2014). Comparative study of antibacterial activity of peel extracts of Citrus aurantium L. (Bitter Orange) and Citrus medica L. (Lemon) against clinical isolates from wound infection. International Journal of Pharma and Bio Sciences, 5(1), 382–387.

28. Willey, J., Sherwood, L., & Woolverton, C. (2011). Prescott Microbiology. New York: McGraw Hill.

29. Goossens, H., Ferech, M., Vander Stichele, R., & Elseviers, M. (2005). Outpatient antibiotic use in Europe and association with resistance: a cross-national database study. The Lancet, 365(9459), 579–587. doi: 10.1016/s0140-6736(05)17907-0

30. Onifade, A. K., Oladoja, M. A., Fadipe, D. O. (2015). Antibiotics Sensitivity Pattern of Escherichia coli isolated from Children of School Age in Ondo State, Nigeria. Researcher, 7(2), 73–76.