Isolation and Identification of Bacterial Species Associated with Cow Milk Sold in Some Selected Areas of Bauchi Metropolis, Bauchi State, Nigeria
Milk is well known to be a balanced diet with high nutritional values. Conversely, milk and milk products may serve as potential substrates for the growth and proliferation of a range of bacteria which in turn fatally influences public health concerns. The study was conducted in 50 milk samples collected from apparently healthy cows during the period for isolation, identification of bacterial isolate based on their morphology, staining, cultural, biochemical properties and gram staining to ascertain their identity. The results indicated that Staphylococcus aureus (34.58 %) has the highest occurrence, followed by Bacillus cereus (19.64 %), followed by Escherichia coli (15.89 %), Salmonella typhi with (13.08 %), Pseudomonas aerugnosa with (7.94 %), Klebsiella pneumonia with (5.14 %).
In comparison, Enterobacter has the most minor occurrence at (3.74 %). The presence of these organisms could be attributed to dirty and unkempt behaviour of the milking process, handling and storing, which in turn will impose serious health hazards to the immediate community and consumers. To reduce the risk associated with these organisms, all personal hygiene measures and materials involved in the milking process should be sterilised and free from any form of organisms before the final approach.
1. Khan, Z. I., Ahmad, K., Bayat, A., Mukhtar M. K., & Sher, M. E. (2013). Valuation of Lead Concentration in Pasture and Milk: A Possible Risk for Livestock and Public Health. Pakistan Journal of Zoology, 45(1), 79–84.
2. Garedew, L., Berhanu, A., Mengesha, D., & Tsegay, G. (2012). Identification of gram-negative bacteria from critical control points of raw and pasteurised cow milk consumed at Gondar town and its suburbs, Ethiopia. BMC Public Health, 12(1). doi: 10.1186/1471-2458-12-950
3. Claeys, W. L., Cardoen, S., Daube, G., De Block, J., Dewettinck, K., Dierick, K., De Zutter, L., Huyghebaert, A., Imberechts, H., Thiange, P., Vandenplas, Y., & Herman, L. (2013). Raw or heated cow milk consumption: Review of risks and benefits. Food Control, 31(1), 251–262. doi: 10.1016/j.foodcont.2012.09.035
4. Fadaei, A. (2014). Bacteriological quality of raw cow milk in Shahrekord, Iran. Veterinary World, 7(4), 240–243. doi: 10.14202/vetworld.2014.240-243
5. Fatima Bouazza. (2012). Hygienic quality of raw milk at Sardi breed of sheep in Morocco. African Journal of Microbiology Research, 6(11). doi: 10.5897/ajmr11.1396
6. Marjan, S., Kanta Das, K., Kishore Munshi, S., & Noor, R. (2014). Drug-resistant bacterial pathogens in milk and some milk products. Nutrition & Food Science, 44(3), 241–248. doi: 10.1108/nfs-05-2013-0061
7. Yasmin, S., Parveen, S., Munna, Md., & Noor, R. (2015). Detection of Salmonella spp. and Microbiological Analysis of Milk and Milk Based Products Available within Dhaka Metropolis, Bangladesh. British Microbiology Research Journal, 5(6), 474–480. doi: 10.9734/bmrj/2015/11010
8. Chen, Y., Jackson, K. M., Chea, F. P., & Schaffner, D. W. (2001). Quantification and Variability Analysis of Bacterial Cross-Contamination Rates in Common Food Service Tasks. Journal of Food Protection, 64(1), 72–80. doi: 10.4315/0362-028x-64.1.72
9. Sherman, N. (2005). Microbiology: A laboratory manual (6th ed.). New York: Pearson/Benjamin Cummings.
10. Boone, D. R., Castenholz, R. W., Garrity, G. M., & Bergey, D. H. (2001). Bergey's manual of systematic bacteriology (2nd ed.). New York: Springer.
11. Cheesbrough, M. (2006). District Laboratory practice in tropical countries (2nd ed.). Cambridge: Cambridge University Press.
12. Cappuccino, J. G., & Sherman, N. (2011). Microbiology: a laboratory manual (9th ed). Boston: Pearson.
13. El-Kosi, O. H. R., Abdel-Hakiem. E. H., & Saad. H. A. (2000). Fate of enterohaemorrhagic Escherichia coli 0157: H7 in buffalo's milk and some of its manufacturing products. Tropenlandwirt, Beiheft, 69, 165–175.
14. Boycheva, S., Dimitrov, T., Tsankova, M., & Iliev, T. (2002). Investigation on Microflora of Buffalo Milk. Bulgarian Journal of Agricultural Science, 8(2/3), 279–282.
15. Stewart, G. C. (2008). Staphylococcus aureus. In P. Fratamico, A. Bhunia & J. Smith, Foodborne Pathogens: Microbiology and Molecular (section 13). N. d.: Caister Academic Press.
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